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Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
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Deep Eutectic Solvent-Based Aqueous Two-Phase Systems and Their Application in Partitioning of Phenol Compounds
Isabela N Souza1, Lucas C V Rodrigues2, Cleide M F Soares1,3
1Post-Graduated Program on Process Engineering, Tiradentes University, Aracaju 49032-490, SE, Brazil.
Molecules (Basel, Switzerland)
|September 28, 2024
Summary
Deep eutectic solvents (DESs) create effective aqueous two-phase systems (ATPSs) for separating phenolic compounds. These DES-based ATPSs demonstrate enhanced partitioning and selective recovery of valuable biomolecules.
Area of Science:
- Green Chemistry
- Separation Science
- Biochemistry
Background:
- Phenolic compounds are valuable biomolecules with diverse applications.
- Traditional separation methods can be inefficient and environmentally impactful.
- Aqueous two-phase systems (ATPSs) offer a promising alternative for biomolecule separation.
Purpose of the Study:
- To investigate the formation and characteristics of ATPSs using deep eutectic solvents (DESs) and acetonitrile.
- To evaluate the partitioning behavior of phenolic compounds (caffeic acid, syringic acid, vanillic acid, ferulic acid, vanillin) in these systems.
- To assess the selectivity and efficiency of DES-based ATPSs for phenolic compound separation.
Main Methods:
- Construction of binodal curves at 25 °C and 0.1 MPa to define the biphasic regions of ATPSs.
- Comparison of DES-based ATPSs with traditional systems using choline chloride ([Ch]Cl) and carbohydrates.
- Partitioning studies of five phenolic compounds in the developed ATPSs.
- Calculation of partition coefficients (K) and recovery percentages in the acetonitrile-rich phase.
Main Results:
- DES-based ATPSs exhibited larger biphasic regions than [Ch]Cl-based systems due to increased hydrophilicity.
- All studied phenolic compounds preferentially partitioned to the acetonitrile-rich phase (K > 1).
- Recovery percentages in the top phase ranged from 53.36% (caffeic acid) to 90.09% (vanillin).
- Selective separation was achieved, with ferulic acid and vanillin favoring the top phase, and syringic, caffeic, and vanillic acids favoring the bottom phase, showing selectivity > 2.
Conclusions:
- DESs are effective in forming stable and efficient ATPSs for phenolic compound separation.
- DES-based ATPSs offer advantages in hydrophilicity and biphasic region size compared to traditional systems.
- These systems provide a selective and efficient method for the recovery of specific phenolic compounds.
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