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Honeysuckle-Derived miR2911 Inhibits Replication of Porcine Reproductive and Respiratory Syndrome Virus by Targeting
Xinyan Cao1, Jiaxi Xue1, Adnan Ali1
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Abstract:
The highly abundant and stable antiviral small RNA derived from honeysuckle, known as miR2911, has been shown to play a key role in inhibiting influenza virus infection and SARS-CoV-2 infection. However, whether miR2911 inhibits the replication of porcine reproductive and respiratory syndrome virus (PRRSV) remains unknown. Hence, this study investigated the mechanisms underlying the action of miR2911 during PRRSV infection. Six targets of miR2911 within the PRRSV orf1 (Nsp2: 2459 to 2477, 1871 to 1892, 954 to 977, and 1271 to 1292; Nsp1: 274 to 296 and 822 to 841) were successfully identified by using the miRanda v1.0b software. The miR2911 target sequence was analyzed by target sequence comparison, and only individual base mutations existed in different prevalent strains, and the miR2911 target region was highly conserved among different strains. Subsequently, through the dual luciferase reporter gene assay and miR2911 overexpression assay, it was demonstrated that miR2911 significantly inhibits the replication of PRRSV by targeting regions of PRRSV Nsp1 and Nsp2. These findings offer new insights for the development of novel anti-PRRSV drugs.
Insights
Honeysuckle-derived miR2911, an antiviral small RNA, effectively inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication. This discovery opens avenues for developing new anti-PRRSV therapies.
Area of Science:
- * Virology
- * Molecular Biology
- * Antiviral Therapeutics
Background:
- * MicroRNA 2911 (miR2911) is a small RNA from honeysuckle with known antiviral properties against influenza and SARS-CoV-2.
- * The antiviral efficacy of miR2911 against porcine reproductive and respiratory syndrome virus (PRRSV) remains uninvestigated.
Purpose of the Study:
- * To investigate the potential of miR2911 in inhibiting PRRSV replication.
- * To elucidate the molecular mechanisms by which miR2911 might act against PRRSV.
Main Methods:
- * Bioinformatic analysis using miRanda v1.0b software to identify potential miR2911 targets in PRRSV orf1.
- * Sequence comparison to assess the conservation of target regions across different PRRSV strains.
- * Dual luciferase reporter gene assays and miR2911 overexpression assays to validate target interactions and functional inhibition.
Main Results:
- * Six potential miR2911 target sites were identified within PRRSV non-structural proteins 1 (Nsp1) and 2 (Nsp2).
- * The identified miR2911 target regions within the PRRSV genome were found to be highly conserved among different strains.
- * miR2911 was demonstrated to significantly inhibit PRRSV replication by targeting specific regions in Nsp1 and Nsp2.
Conclusions:
- * miR2911 possesses significant antiviral activity against PRRSV by directly targeting viral Nsp1 and Nsp2 proteins.
- * The high conservation of target sites suggests miR2911's broad applicability against various PRRSV strains.
- * These findings provide a foundation for developing novel therapeutic strategies targeting miR2911 for PRRSV control.
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