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Proteomics

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Updated: Jun 11, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Multiplexed Target Profiling with Integrated Chemical Genomics and Chemical Proteomics.

Yang Yang^1,2, Yin-Suen Tse^1,3, Qi Zhang²

¹State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, SAR 999077, China.

Journal of Medicinal Chemistry

|September 28, 2024

Summary

This summary is machine-generated.

This study introduces chemical genomics-facilitated chemical proteomics (CGCP), a new method for identifying drug targets. CGCP efficiently identifies multiple targets for various bioactive molecules simultaneously, accelerating drug discovery.

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Area of Science:

Proteomics
Chemical Genomics
Drug Discovery

Background:

Target identification is essential for understanding bioactive molecules in drug discovery.
Current methods are inefficient for analyzing multiple, structurally diverse compounds simultaneously.

Purpose of the Study:

To develop a novel strategy for multiplexed target identification of bioactive small molecules.
To enhance the efficiency of identifying targets for structurally distinct compounds.

Main Methods:

Developed chemical genomics-facilitated chemical proteomics (CGCP) for simultaneous target identification.
Correlated chemical genomic profiles (global transcription perturbations) with protein reactivity.
Applied CGCP to celastrol and four other electrophilic compounds.

Main Results:

Identified multiple novel targets and binding sites for the tested compounds in a single experiment.
Demonstrated CGCP's ability to handle structurally diverse compounds.
Showcased multiplexing capability for efficient target identification.

Conclusions:

CGCP significantly improves the efficiency of target identification for multiple, structurally distinct bioactive molecules.
This method has the potential to accelerate the drug discovery process.
CGCP enables simultaneous target identification, overcoming limitations of traditional methods.