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Updated: Jul 15, 2026

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
Detrended fluctuation analysis to determine physiologic thresholds, investigation and evidence from incremental
Johan Cassirame1,2,3, Esther Eustache4, Lucas Garbellotto5
1Laboratory Culture Sport Health and Society (C3S-UR 4660, Sport and Performance Department, University of Franche-Comte, 25000, Besançon, France. johancassirame@free.fr.
The detrend fluctuation analysis (DFA α1) method showed poor agreement for determining ventilatory thresholds (VT1 and VT2) in well-trained cyclists. This indirect heart rate variability (HRV) approach is not a reliable substitute for gas-exchange thresholds (GET).
Area of Science:
- Exercise Physiology
- Sports Science
- Biomedical Engineering
Background:
- Gas-exchange thresholds (GET) are standard for assessing training zones.
- Heart rate variability (HRV) analysis offers potential for indirect GET determination.
- Detrend fluctuation analysis (DFA α1) is one such HRV method.
Purpose of the Study:
- To evaluate the agreement between DFA α1 and ventilatory thresholds (VT1 and VT2) in well-trained cyclists.
- To assess the reliability of DFA α1 for determining GET compared to traditional methods.
Main Methods:
- Eighteen female and 38 male sub-elite cyclists underwent maximal incremental cycling tests.
- Continuous gas exchange and heart rate (HR) were measured.
- Agreements between DFA α1-derived thresholds (HRVT1, HRVT2) and ventilatory thresholds (VT1, VT2) were analyzed using Bland-Altman analysis for power output (PO), oxygen uptake (VO2), and HR.
Main Results:
- Significant and large limits of agreement were found between VT1 and HRVT1 for VO2, HR, and PO.
- Low agreement was also observed between VT2 and HRVT2 for VO2, HR, and PO.
- Women showed poorer predictions from DFA α1 compared to men for HR and PO at VT1 and VT2.
Conclusions:
- The DFA α1 method demonstrates unsatisfactory agreement for determining VT1 and VT2 in well-trained cyclists of both sexes.
- DFA α1 is not a reliable indirect method for GET determination in this population.
- Further research may be needed to refine HRV analysis for accurate training zone prescription.
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