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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Bridging basic science and applied diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic

Anna Nele Herdina1, Anil Bozdogan2, Patrik Aspermair3

  • 1Department of Laboratory Medicine, Division of Clinical Virology, Medical University of Vienna, Vienna, Austria.

Biosensors & Bioelectronics
|September 28, 2024
PubMed
Summary

A novel graphene field-effect transistor (gFET) biosensor rapidly detects SARS-CoV-2 RNA and nucleocapsid protein. This sensitive, point-of-care diagnostic tool aids in assessing viral infectiousness and managing future pandemics.

Keywords:
COVID-19DiagnosticsGraphene field-effect transistorInfectivityNucleocapsid proteinSARS-CoV-2

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Infectious Disease Diagnostics

Background:

  • Accurate and rapid diagnostics are crucial for managing infectious diseases like SARS-CoV-2.
  • Existing methods like RT-PCR can be time-consuming and require specialized facilities.
  • A need exists for point-of-care (POC) devices that can assess both viral presence and infectiousness.

Purpose of the Study:

  • To develop and validate a graphene field-effect transistor (gFET) biosensor for dual detection of SARS-CoV-2.
  • To assess viral RNA for positivity and nucleocapsid (N-)protein as a proxy for infectiousness.
  • To evaluate the gFET biosensor's potential as a POC device for SARS-CoV-2 diagnosis and infectivity assessment.

Main Methods:

  • A gFET biosensor was engineered to detect both viral RNA (targeting the E-gene) and SARS-CoV-2 N-protein using specific antibodies.
  • Clinical nasopharyngeal swab samples (n=213) were tested for SARS-CoV-2 RNA and correlated with RT-PCR cycle threshold values.
  • N-protein detection was assessed for its limit of detection (LOD) and correlation with virus culture results (n=16).

Main Results:

  • The gFET biosensor demonstrated high sensitivity in detecting SARS-CoV-2 RNA, correlating well with RT-PCR values.
  • Specificity was confirmed against pre-pandemic samples containing other respiratory viruses.
  • The N-protein assay achieved an LOD of 0.9 pM and showed strong correlation with virus culture, indicating infectiousness.

Conclusions:

  • The gFET biosensor offers a sensitive and rapid method for diagnosing SARS-CoV-2 infection.
  • Dual detection of RNA and N-protein provides a comprehensive assessment of viral presence and infectiousness.
  • This technology holds promise as a POC device for improved clinical and public health decision-making during pandemics.