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Updated: Jun 11, 2025
Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates
Published on: February 24, 2011
A time-saving one-step polyacrylamide gel with a colored stacking gel for SDS-PAGE and western blotting
1Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, 471 Ebigase, Higashi-ku, 950-8680, Niigata, Japan.
Abstract:
A time-saving, one-step polyacrylamide gel preparation method enabling simultaneous preparation of separating and stacking gels was previously reported, but the boundary between the separating and stacking gels was often not well defined. As such, determining whether the gel preparation failed is difficult before SDS-PAGE is carried out. To address this issue, a one-step polyacrylamide gel preparation method was developed in which the stacking gel is colored to allow better visualization of the border between the stacking and separating gels. This new one-step method saves time and achieves comparable performance for SDS-PAGE and western blotting to that obtained with conventional gels.
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