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Related Experiment Videos

Occluding junction structure-function relationships in a cultured epithelial monolayer.

J L Madara, K Dharmsathaphorn

    The Journal of Cell Biology
    |December 1, 1985
    PubMed
    Summary
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    Electrical circuit analysis of occluding junctions (OJs) in T84 cells reveals structural correlates to ion flow resistance. Analyzing OJ strand counts as parallel resistors accurately predicts transepithelial resistance development.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Epithelial Physiology

    Background:

    • Occluding junctions (OJs) form the primary seal in epithelial cell layers, controlling paracellular transport.
    • Understanding the relationship between OJ structure and barrier function is crucial for various physiological processes.

    Purpose of the Study:

    • To investigate the structural development of occluding junctions in T84 cells.
    • To correlate occluding junction structure with the development of transepithelial electrical resistance.
    • To determine if electrical circuit analysis of OJ structure can predict barrier function.

    Main Methods:

    • Cultured T84 cell monolayers were used to study OJ development.
    • Electrical circuit analysis was applied to OJ strand count distribution histograms.

    Related Experiment Videos

  • Transepithelial resistance was measured, and unidirectional solute fluxes were determined.

    • Main Results:

    • Electrical circuit analysis of OJ strand counts predicted transepithelial resistance increments (1:21:50) that closely matched measured values (1:29:59).
    • Analysis failed when using mean strand counts or a different strand count-resistance relationship.
    • Restriction of transjunctional permeability, not cell membrane resistance, accounted for the observed resistance rise.

    Conclusions:

    • Structural correlates between occluding junction morphology and passive ion flow resistance exist in T84 monolayers.
    • Analyzing OJ structural data as an electrical circuit of parallel resistors is essential for obtaining these correlates.
    • T84 junctional strands exhibit selective permeability, restricting larger molecules while allowing passage of smaller ions.