Related Experiment Video
Updated: Jun 11, 2025
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Development of a ratiometric fluorescent probe based on caffeic acid for hydrazine detection and its applications in
Haoxue Tan1, Zhonglong Wang2, Xiaoqin Yang1
1Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, PR China.
Abstract:
Hydrazine (N2H4) has been extensively utilized as a highly reactive chemical reagent. However, it is also seriously harmful to human beings and ecosystem. Thus, the development of an efficient detecting method for hydrazine is desirable. Here, caffeic acid was chose as starting material to synthesize a new ratiometric fluorescent probe HPA for detecting hydrazine. This probe possessed the specific recognition ability for hydrazine over other analytes with low detection limit (0.106 µM) and extremely short time (60 s). The sensing mechanism of probe HPA for hydrazine was proved by 1H NMR titration and theoretical calculations. In addition, the probe HPA was loaded on paper strip for rapid quantitative detection of hydrazine with the aid of a software (Image J). The effective detecting performances of probe HPA for hydrazine were verified in environmental water samples as well as in living cells. Thus, HPA has great potential for detection and analysis of hydrazine in health supervision and environmental protection.
