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Related Experiment Video

Updated: Jun 11, 2025

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

Profiling Long Noncoding RNA in Psoriatic Skin Using Single-Cell RNA Sequencing.

Rachael Bogle¹, Matthew T Patrick¹, Sutharzan Sreeskandarajan²

¹Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA.

The Journal of Investigative Dermatology

|September 29, 2024

Summary

This summary is machine-generated.

Single-cell RNA sequencing successfully profiled thousands of skin-expressed long noncoding RNAs (lncRNAs) in psoriasis patients. This method identified cell-type-specific lncRNAs and revealed AC020916.1

Keywords:

Bioinformatics Keratinocytes LncRNA Psoriasis Single-cell RNA-seq

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Area of Science:

Dermatology
Genomics
Molecular Biology

Background:

Long noncoding RNAs (lncRNAs) roles in epidermal differentiation are known from bulk RNA sequencing.
Most lncRNAs lack single-cell level annotation despite tissue-specific expression.

Purpose of the Study:

To evaluate single-cell RNA sequencing (scRNA-seq) for profiling skin lncRNAs.
To characterize lncRNAs and their cellular sources in psoriatic skin.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) on paired uninvolved and lesional psoriatic skin from 6 patients.
Differential gene expression analysis and spatial transcriptomics validation.

Main Results:

Detected over 7000 skin-expressing lncRNAs and identified their cellular origins.
Identified 137 differentially expressed lncRNAs in lesional psoriatic skin.
Found keratinocytes express the most differentially expressed lncRNAs, including AC020916.1, which correlates with proliferation/differentiation genes.

Conclusions:

scRNA-seq is effective for profiling skin lncRNAs and inferring cellular origins.
This approach can advance the study of inflammatory skin conditions.