JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

MIEN1 on the 17q12 amplicon facilitates the malignant behaviors of gastric cancer via activating IL-6/JAK2/STAT3 pathway

  • 0Scientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
The International Journal of Biochemistry & Cell Biology +

|

September 29, 2024

|

September 29, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Migration and invasion enhancer 1 (MIEN1) amplification drives gastric cancer progression by activating the IL-6/JAK2/STAT3 pathway. Targeting MIEN1 offers a promising therapeutic strategy for advanced gastric cancer patients.

Area Of Science

  • Oncology
  • Genetics
  • Molecular Biology

Background

  • Oncogene amplification is a key driver of poor prognosis in advanced gastric cancer.
  • Identifying novel therapeutic targets is crucial for improving patient outcomes.

Purpose Of The Study

  • To investigate the role of amplified oncogenes, specifically MIEN1, in gastric cancer progression.
  • To elucidate the underlying molecular mechanisms of MIEN1-driven gastric cancer.

Main Methods

  • Array comparative genomic hybridization (aCGH) to identify amplified regions.
  • Analysis of MIEN1 copy number and expression in gastric cancer cell lines and patient tissues.
  • In vitro functional assays (proliferation, migration, invasion) and in vivo xenograft models.
  • Western blot analysis to assess signaling pathway activation.

Main Results

  • High amplification and overexpression of MIEN1 were identified in gastric cancer, correlating with sex and Lauren's intestinal type.
  • Elevated MIEN1 expression was associated with poorer patient survival.
  • MIEN1 overexpression enhanced proliferation, invasion, and migration; knockdown reversed these effects.
  • MIEN1 activates the IL-6/JAK2/STAT3 signaling pathway, promoting gastric cancer cell malignancy.
  • MIEN1 knockdown inhibited tumor growth and metastasis in vivo.

Conclusions

  • MIEN1 is a significant oncogene amplified in gastric cancer, contributing to malignant phenotypes.
  • MIEN1 promotes gastric cancer progression via the IL-6/JAK2/STAT3 pathway.
  • MIEN1 represents a potential therapeutic target for gastric cancer treatment.

Keywords:

Related Concept Videos

mTOR Signaling and Cancer Progression 03:03

3.8K

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation 02:23

4.5K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Induced Pluripotent Stem Cells 01:06

4.0K

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...