Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA sensors and actuators for dynamic cellular regulation.

Trends in biotechnology·2026
Same author

Peroxisome Engineering of Yarrowia lipolytica for Fatty Alcohol Production.

Biotechnology and bioengineering·2026
Same author

Protein-Based Nanostructures: Column-free Biosynthesis of Bundlemer Peptides with Programmable, Orthogonally Reactive Handles for Nanomaterial Construction.

ACS applied materials & interfaces·2025
Same author

Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation.

Nucleic acids research·2025
Same author

MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells.

iScience·2025
Same author

Synthetic protein degradation circuits using programmable cleavage and ligation by Sortase A.

Nature communications·2025
Same journal

Enzymes for CO<sub>2</sub> fixation: Discovery, engineering, and applications.

Biotechnology advances·2026
Same journal

Technological advances in extrachromosomal circular DNA detection.

Biotechnology advances·2026
Same journal

Codon compression and novel codon creation for multiplex non-canonical amino acid incorporation.

Biotechnology advances·2026
Same journal

Toward next-generation biosurfactants: Engineering rhamnolipid production from safe chassis design to scalable bioprocessing.

Biotechnology advances·2026
Same journal

Advances and challenges in alternative proteins: From biotechnology to sustainable food production.

Biotechnology advances·2026
Same journal

Recent advances in the microbial production of L-arginine and its derivatives using engineering Corynebacterium glutamicum and Escherichia coli.

Biotechnology advances·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2025

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.3K

Engineering conditional protein-protein interactions for dynamic cellular control.

Anthony M Stohr1, Derron Ma1, Wilfred Chen1

  • 1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.

Biotechnology Advances
|September 29, 2024
PubMed
Summary
This summary is machine-generated.

Engineered conditional protein-protein interactions offer dynamic cellular control for therapeutics and metabolic engineering. This review highlights computational tools to accelerate the design of novel, stimulus-responsive protein interactions.

Keywords:
Computational protein designDynamic regulationProtein engineeringProtein-protein interactions

More Related Videos

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

8.2K
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

2.9K

Related Experiment Videos

Last Updated: Jun 11, 2025

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.3K
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

8.2K
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

2.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Conditional protein-protein interactions (CPPIs) are crucial for regulating cellular processes.
  • CPPIs are engineered to respond to external stimuli like chemicals or light.
  • Applications include assembling protein fragments, building scaffolds, and organizing proteins.

Purpose of the Study:

  • To provide an overview of designing novel engineered protein interactions.
  • To showcase computational tools that accelerate protein engineering.
  • To foster development of CPPIs responding to new inputs or in alternative settings.

Main Methods:

  • Review of existing literature on engineered protein interactions.
  • Highlighting computational tools for protein design and engineering.
  • Discussion of stimuli-responsive interaction design.

Main Results:

  • CPPIs can be engineered to respond to diverse stimuli.
  • Existing computational tools can accelerate the design process.
  • Successful applications demonstrated in various host systems.

Conclusions:

  • Engineered CPPIs are versatile tools for biological applications.
  • Computational approaches are key to advancing CPPI design.
  • Further development of novel CPPIs is encouraged for expanded applications.