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Related Experiment Videos

IncP-1 R plasmids decrease the serum resistance and the virulence of Pseudomonas aeruginosa.

B Wretlind, K Becker, D Haas

    Journal of General Microbiology
    |October 1, 1985
    PubMed
    Summary

    IncP-1 R plasmids significantly reduced Pseudomonas aeruginosa virulence and increased susceptibility to human serum. Virulence was restored by plasmid curing, highlighting the plasmid

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    Area of Science:

    • Microbiology
    • Bacterial Pathogenesis
    • Molecular Biology

    Background:

    • Pseudomonas aeruginosa is an opportunistic pathogen.
    • R plasmids are a class of mobile genetic elements.
    • The impact of R plasmids on bacterial virulence is not fully understood.

    Purpose of the Study:

    • To investigate the effect of IncP-1 R plasmids on Pseudomonas aeruginosa virulence.
    • To determine if R plasmids alter bacterial susceptibility to host immune defenses.

    Main Methods:

    • Experimental mouse burn infection model.
    • Determination of mean lethal dose (LD50).
    • Assessment of bacterial sensitivity to human serum bactericidal activity.

    Main Results:

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    • All tested R plasmids (RP1, R68, R68.45) increased the LD50 of P. aeruginosa PAO1 by 10- to 400-fold.
    • Plasmids R68 and R68.45 caused a more significant decrease in virulence than RP1.
    • All plasmids increased bacterial sensitivity to human serum.
    • Curing of R68.45 restored virulence and serum resistance, unlike deletions in transfer genes.

    Conclusions:

    • IncP-1 R plasmids significantly attenuate the virulence of Pseudomonas aeruginosa.
    • R plasmids confer increased susceptibility to serum bactericidal activity.
    • The entire plasmid, not just transfer genes, is crucial for the observed effects on virulence and serum resistance.