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  1. Home
  3. Temporomandibular Joint Pain Measurement By Bite Force And Von Frey Filament Assays In Mice.
  1. Home
  3. Temporomandibular Joint Pain Measurement By Bite Force And Von Frey Filament Assays In Mice.

Related Experiment Video

Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice
06:37

Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice

Published on: September 13, 2024

879

Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice.

Supawadee Jariyasakulroj1, Qing Chang2, Pao-Fen Ko2

  • 1Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles; Department of Masticatory Science, Faculty of Dentistry, Mahidol University.

Journal of Visualized Experiments : Jove
|September 30, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a reliable mouse model for temporomandibular joint (TMJ) osteoarthritis pain using Complete Freund

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Area of Science:

  • Biomedical Sciences
  • Pain Research
  • Osteoarthritis Studies

Background:

  • Temporomandibular joint (TMJ) disorders, including pain and osteoarthritis (OA), significantly reduce patient quality of life.
  • Current understanding of disease mechanisms and effective long-term treatments for TMJ disorders remains limited.
  • Accurate pain assessment in orofacial pain research presents significant challenges for both clinical and basic science studies.

Purpose of the Study:

  • To establish a robust inflammatory temporomandibular joint (TMJ) pain mouse model.
  • To evaluate the efficacy of established pain assessment methods in this model.
  • To provide reliable tools for future TMJ pain research.

Main Methods:

  • An inflammatory TMJ pain mouse model was created using intra-articular injection of Complete Freund's Adjuvant (CFA).
  • Orofacial pain behaviors were assessed using bite force measurements.
  • Mechanical allodynia was evaluated using the von Frey filament test.

    • Main Results:

    • Mice receiving CFA injections demonstrated significant orofacial pain behaviors compared to controls.
    • Reduced bite force was observed in the CFA-injected group.
    • A decreased head withdrawal threshold in the von Frey filament test indicated mechanical allodynia.

    Conclusions:

    • Bite force measurement and the von Frey filament test are reliable and reproducible methods for assessing orofacial pain.
    • The developed CFA-induced TMJ OA mouse model effectively mimics painful TMJ disorders.
    • These validated methods can be applied to future studies on TMJ disorders and other pain models.