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Published on: June 11, 2015
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Complete genome sequence of Mycobacteriophage Eaglepride
Umar Sahi1, Niati G Kottury2, Nirav R Kottury2
1Department of Biology, University of Central Oklahoma, Edmond, Oklahoma, USA.
Microbiology Resource Announcements
|September 30, 2024
Summary
Researchers isolated the Eaglepride mycobacteriophage using Mycobacterium smegmatis. This phage, assigned to subcluster A10, shows high genomic similarity to other actinobacteriophages, particularly OKCentral2016.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Mycobacteriophages are viruses that infect mycobacteria.
- Understanding mycobacteriophage diversity is crucial for developing novel therapeutic strategies against mycobacterial infections.
- Mycobacterium smegmatis is a commonly used model organism in mycobacteriology research.
Purpose of the Study:
- To isolate and characterize a novel mycobacteriophage.
- To determine the genomic features of the isolated phage.
- To classify the phage based on its genetic relatedness to known phages.
Main Methods:
- Isolation of mycobacteriophage using enrichment culture with Mycobacterium smegmatis mc²155.
- Genome sequencing and analysis to determine genome length, open reading frames (ORFs), and tRNA genes.
- Comparative genomic analysis to determine phage classification and nucleotide identity with related phages.
Main Results:
- The mycobacteriophage, named Eaglepride, was successfully isolated.
- Eaglepride possesses a genome of 50,926 bp, containing 89 open reading frames and 1 tRNA gene.
- Phylogenetic analysis based on gene content places Eaglepride in subcluster A10 of actinobacteriophages, with highest nucleotide identity to OKCentral2016.
Conclusions:
- Eaglepride represents a new addition to the known diversity of mycobacteriophages.
- Its genomic characteristics and phylogenetic placement provide insights into the evolution and classification of actinobacteriophages.
- Further studies on Eaglepride may reveal its potential applications in phage therapy or as a research tool.
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