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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Jun 11, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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Recent advances in the CRISPR/Cas system-based visual detection method.

Jinrong Chen1,2, Hang Su2, June Hyun Kim1

  • 1Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, Korea. liurcau@suwon.ac.kr.

Analytical Methods : Advancing Methods and Applications
|September 30, 2024
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR)/associated nucleases (Cas) offer rapid, sensitive visual detection for environmental threats. This review explores CRISPR-based diagnostic methods, focusing on visual readouts for point-of-care applications.

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Area of Science:

  • Molecular Diagnostics
  • Biotechnology
  • Environmental Health

Background:

  • Infectious pathogens, toxins, and heavy metals threaten global health and economies.
  • Rapid, sensitive, and convenient visual molecular detection methods are urgently needed.
  • CRISPR/Cas systems have emerged as a powerful tool for molecular diagnostics.

Purpose of the Study:

  • To review recent developments in CRISPR/Cas-based visual detection methods.
  • To analyze the advantages and disadvantages of different CRISPR/Cas effectors for diagnostics.
  • To discuss challenges and future prospects in CRISPR/Cas visual detection.

Main Methods:

  • Review of CRISPR/Cas effectors and their diagnostic principles.
  • Focus on visual readout signals in point-of-care testing (POCT).
  • Analysis of colorimetric, fluorescence, and lateral flow assays.

Main Results:

  • CRISPR/Cas technology enables diverse visual detection strategies.
  • Different CRISPR/Cas systems offer varying diagnostic capabilities.
  • Visual readouts are crucial for accessible point-of-care diagnostics.

Conclusions:

  • CRISPR/Cas-based visual detection holds significant promise for rapid diagnostics.
  • Further research is needed to overcome current challenges and expand applications.
  • These methods can address global health and environmental monitoring needs.