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Updated: Jun 11, 2025

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
09:30

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Published on: January 13, 2018

7.8K

YAP/TAZ signaling in allantois-derived cells is required for placental vascularization

Siqi Gao, Triloshan Thillaikumaran, Martin H Dominguez

    Biorxiv : the Preprint Server for Biology
    |September 30, 2024
    PubMed

    Normal placental development and angiogenesis are crucial for fetal growth and maternal health during pregnancy. However, molecular regulation of placental angiogenesis has been difficult to study due to a lack of specific genetic tools that isolate the placenta from the embryo and yolk sac. To address this gap in knowledge we recently developed Hoxa13 Cre mice in which Cre is expressed in allantois-derived cells, including placental endothelial and stromal cells. Mice lacking the transcriptional regulators Yes-associated protein (YAP) and PDZ-binding motif (TAZ) in allantois-derived cells exhibit embryonic lethality at midgestation with compromised placental vasculature. snRNA-seq analysis revealed transcriptional changes in placental stromal cells and endothelial cells. YAP/TAZ mutants exhibited significantly reduced placental stromal cells prior to the endothelial architectural change, highlighting the role of these cells in placental vascular growth. These results reveal a central role for YAP/TAZ signaling during placental vascular growth and implicate Hoxa13 -derived placental stromal cells as a critical component of placental vascularization.

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