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Hyperpolarizing membrane potential changes in a cloned monkey kidney cell line.

H Chang, N Yamashita, E Ogata

    Pflugers Archiv : European Journal of Physiology
    |October 1, 1985
    PubMed
    Summary
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    This study investigated electrophysiological properties of monkey kidney cells (JTC-12), revealing spontaneous and induced hyperpolarizations. These events are linked to increased cell membrane permeability to potassium ions.

    Area of Science:

    • Cellular Electrophysiology
    • Renal Cell Biology

    Background:

    • Understanding kidney cell electrophysiology is crucial for renal function.
    • The JTC-12 cell line provides a model for studying monkey kidney cell properties.

    Purpose of the Study:

    • To characterize the electrophysiological properties of the JTC-12 monkey kidney cell line.
    • To investigate the mechanisms underlying spontaneous and induced hyperpolarizations in these cells.

    Main Methods:

    • Electrophysiological recordings using patch-clamp techniques.
    • Measurement of resting membrane potential, input resistance, and current-voltage relationships.
    • Stimulation via mechanical and electrical means.

    Main Results:

    • JTC-12 cells exhibited a resting potential of -15.3 mV and input resistance of 78 MΩ.

    Related Experiment Videos

  • Spontaneous and stimulus-evoked hyperpolarizations were observed, associated with increased membrane conductance.
  • The reversal potential for hyperpolarization was -72.7 mV, and it occurred in chloride-free solutions.

    • Conclusions:

    • JTC-12 cells display spontaneous and induced hyperpolarizations.
    • These hyperpolarizations are primarily mediated by an increase in membrane permeability to potassium ions.