Effect of ethanol on prostacyclin and thromboxane A2 synthesis in rat aortic rings in vitro

Spontaneous 6-keto-PGF1 alpha and TXB2 levels in isolated rat aortic rings increased in a concentration dependent manner after a 0.5 hour incubation with moderate or high ethanol concentrations (11 mM to 218 mM). After a 1 hour incubation with moderate concentrations of ethanol less than or equal to 22 mM) spontaneous prostaglandin (PG) production did not increase although high concentrations (87 mM and 218 mM) increased both 6-keto-PGF1 alpha and TXB2 levels. Similarly, in the presence of 40 microM Na-arachidonate, high ethanol concentrations increased PG production after 0.5 and 1 hour incubation. In addition, either a 4 or an 8 hour exposure to high ethanol concentrations increased spontaneous PG production. A moderate concentration of ethanol (22 mM) increased the 6-keto-PGF1 alpha/TXB2 ratio whereas high levels (greater than or equal to 87 mM) depressed the ratio after 0.5 and 1 hour exposure. This effect was short-lived since after 4 or 8 hours incubation with high ethanol concentrations the 6-keto-PGF1 alpha/TXB2 ratio was markedly increased. The alcohol-induced changes in both spontaneous and arachidonate-stimulated PG levels were concentration dependent and related to the incubation time. Furthermore, these data suggest that there may be unbalanced production of PGI2 and thromboxane A2 in vascular tissue exposed to alcohol.