Protein inhibitor of activated signal transducer and activator of transcription 2 is an oncoprotein in oral squamous cell carcinoma and related to cigarette smoking - An in vitro study
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View abstract on PubMed
Summary
This summary is machine-generated.Protein inhibitor of activated signal transducer and activator of transcription 2 (PIAS2) acts as an oncogene in oral cancer. Cigarette smoke condensate increases PIAS2 expression, enhancing oral cancer cell malignancy and impacting survival rates.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Research
Background
- Oral cancer is a prevalent malignancy in Taiwan with a low five-year survival rate (50%) due to cellular heterogeneity.
- Post-translational modifications influence protein diversity and cell function, but the role of PIAS2 in oral cancer is not well understood.
Purpose Of The Study
- To investigate the role of PIAS2 in oral cancer progression.
- To determine the effect of PIAS2 on oral cancer cell malignant behaviors.
- To examine the influence of cigarette smoke condensate (CSC) on PIAS2 expression in oral squamous cell carcinoma (OSCC).
Main Methods
- PIAS2 expression was modulated using overexpression vectors and siRNA knockdown in OSCC cell lines.
- Invasion, migration, and proliferation assays were performed.
- Epithelial-mesenchymal transition (EMT) markers were analyzed.
- The effect of CSC on PIAS2 expression was evaluated.
Main Results
- PIAS2 overexpression significantly enhanced proliferation, invasion, and migration in OSCC cells.
- PIAS2 modulated EMT markers, suppressing E-cadherin and increasing fibronectin.
- PIAS2 knockdown suppressed malignant behaviors and reversed EMT markers.
- CSC treatment dose-dependently increased PIAS2 expression in OSCC cells.
Conclusions
- PIAS2 functions as an oncogene in oral cancer.
- Cigarette smoking induces PIAS2 expression, contributing to oral cancer malignancy.
- Elevated PIAS2 levels are associated with increased oral cancer progression.
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