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Published on: September 20, 2016
The many functions of carbohydrate-active enzymes in family GH65: diversity and application
Emma De Beul1, Jorick Franceus1, Tom Desmet2
1Department of Biotechnology, Centre for Synthetic Biology (CSB), Ghent University, Ghent, Belgium.
Abstract:
Glycoside Hydrolase family 65 (GH65) is a unique family of carbohydrate-active enzymes. It is the first protein family to bring together glycoside hydrolases, glycoside phosphorylases and glycosyltransferases, thereby spanning a broad range of reaction types. These enzymes catalyze the hydrolysis, reversible phosphorolysis or synthesis of various α-glucosides, typically α-glucobioses or their derivatives. In this review, we present a comprehensive overview of the diverse reaction types and substrate specificities found in family GH65. We describe the determinants that control this remarkable diversity, as well as the applications of GH65 enzymes for carbohydrate synthesis.
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