Success rates with preimplantation genetic testing for aneuploidy in good prognosis patients are dependent on age

To evaluate cumulative live birth after preimplantation genetic testing for aneuploidy (PGT-A) with next generation sequencing (NGS) compared with morphology alone among patients aged 21-40 years undergoing single blastocyst transfer.

Retrospective cohort study.

Patients aged 21-40 years undergoing first autologous retrieval cycles resulting in ≥5 fertilized oocytes, with subsequent single blastocyst transfer in Society for Assisted Reproductive Technology clinics from 2016-2019.

PGT-A using NGS.

The primary outcome was cumulative live birth per retrieval. Secondary outcomes included clinical pregnancy, miscarriage, and live birth per transfer.

A total of 56,469 retrieval cycles were included in the analysis. Retrieval cycles were stratified based on age (<35, 35-37, and 38-40 years) and exposure to PGT-A with NGS. Modified Poisson regression modeling was used to evaluate the association between PGT-A and cumulative live birth per retrieval while controlling for covariates. In this cohort, most cycles did not use PGT-A (n = 49,608; 88%). After adjusting for covariates, the use of PGT-A was associated with a slightly lower cumulative live birth in individuals aged <35 years (risk ratio [RR]: 0.96; 95% CI: 0.93-0.99) compared with no PGT, but higher cumulative live birth in ages 35-37 years (RR: 1.04; 95% CI: 1.00-1.08), and 38-40 years (RR: 1.14; 95% CI: 1.07-1.20). A subgroup analysis limited to freeze-all cycles (n = 29,041) showed that PGT-A was associated with higher cumulative live birth in individuals aged ≥35 years and was similar to no PGT in individuals aged <35 years. Miscarriage was significantly less likely in individuals aged ≥35 years using PGT-A compared with no PGT-A.

In this large national database study, success rates in cycles using PGT-A were dependent on age. Cumulative live birth was observed to be significantly less likely in PGT-A cycles among individuals aged <35 years and more likely among individuals aged 38-40 years, compared with no PGT-A. In individuals with no fresh transfer, results were similar. Moreover, miscarriage was significantly less likely with PGT-A among individuals aged 35-40 years in a subgroup analysis of freeze-all cycles.