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The critical role of Gαi3 in oral squamous cell carcinoma cell growth

Quan Li1, Zhiyue Huang2, Zihan Li2

  • 1Department of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou, China. liquan232@163.com.

Cell Death Discovery
|September 30, 2024
PubMed

Insights

G protein inhibitory subunit 3 (Gαi3) is upregulated in oral squamous cell carcinoma (OSCC), driving tumor growth. Inhibiting Gαi3 suppressed OSCC progression and induced apoptosis, highlighting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Oral squamous cell carcinoma (OSCC) requires novel therapeutic targets for effective treatment.
  • G protein inhibitory subunit 3 (Gαi3) is implicated in various cellular processes.
  • Understanding Gαi3's role in OSCC is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the expression, function, and mechanism of G protein inhibitory subunit 3 (Gαi3) in oral squamous cell carcinoma (OSCC).
  • To evaluate Gαi3 as a potential therapeutic target for OSCC treatment.

Main Methods:

  • Gαi3 expression analysis in OSCC tissues and cells.
  • Gαi3 knockdown using shRNA and knockout using CRISPR/Cas9 in OSCC cells.
  • Gαi3 overexpression studies in OSCC cells.
  • Western blot analysis to assess Akt-mTOR pathway activation.
  • In vivo studies using subcutaneous OSCC xenografts in nude mice.

Main Results:

  • Gαi3 was significantly upregulated in OSCC tissues and cells.
  • Gαi3 silencing or knockout inhibited OSCC cell proliferation, migration, and induced apoptosis.
  • Gαi3 overexpression promoted OSCC cell growth, proliferation, and migration.
  • Gαi3 activates the Akt-mTOR signaling pathway in OSCC.
  • Gαi3 silencing suppressed tumor growth in vivo and inactivated the Akt-mTOR pathway.

Conclusions:

  • G protein inhibitory subunit 3 (Gαi3) is a critical driver of OSCC progression.
  • Gαi3 promotes OSCC growth by activating the Akt-mTOR signaling pathway.
  • Gαi3 represents a promising therapeutic target for oral squamous cell carcinoma.

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