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Kill two birds with one stone: colorimetric/fluorescence immunosensor based on Au@Pt nanozyme for sensitive detection
Rongqi Zhai1, Ge Chen2, Beibei Liu1,3
1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Beijing, 100081, China.
Abstract:
Gold-platinum (Au@Pt) nanozymes with high catalytic activity and stability were designed to improve the stability of the enzyme-linked immunosorbent assay (ELISA), and a two-mode signal output was used to enhance the sensitivity and confidence of the assay. This study reports the two-mode signal output based on Au@Pt nanozyme to catalyzed 3,3',5,5'-tetramethylbenzidine (TMB) reaction. Oxidized 3,3',5,5'-tetramethylbenzidine (ox-TMB) has wide absorption spectrum, providing excellent optical density capabilities and fluorescence quenching. The detection limits of imidacloprid were 0.88 μg/L and 1.14 μg/L in colorimetric and fluorescence modes, respectively. Multiple-mode strategy improves detection accuracy, increases the confidence of experimental results, and broadens detection modes. Two modes can meet the requirements of accurate and flexible multi-mode sensing in different application situations.
