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Bergmeyer Glucose Quantification for Microbiological Samples

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

A new method for quantifying glyoxalase II activity in biological samples.

Mohammed Alaa Kadhum¹, Mahmoud Hussein Hadwan¹

¹Department of Chemistry, College of Science, University of Babylon, Hillah City 51002, Iraq.

Biology Methods & Protocols

|October 1, 2024

Summary

A new 2,4-dinitrophenylhydrazine (2,4-DNPH) assay accurately measures Glyoxalase II (Glo II) activity. This simple, cost-effective method offers a reliable alternative for Glo II enzyme research and diagnostics.

Keywords:

Bland–Altman plot DNPH method glyoxalase system medical application methylglyoxal

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Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Published on: May 27, 2015

Area of Science:

Biochemistry
Enzymology

Background:

Glyoxalase II (Glo II) is essential for detoxifying harmful metabolites and maintaining cellular redox balance.
Dysregulation of Glo II is implicated in diseases like cancer and diabetes.

Purpose of the Study:

To develop and validate a novel, cost-effective method for measuring Glyoxalase II activity using 2,4-dinitrophenylhydrazine (2,4-DNPH).

Main Methods:

The assay utilizes the reaction between 2,4-DNPH and pyruvate, a product of the Glo II-catalyzed reaction, to form a colored hydrazone complex.
Absorbance is measured at 430 nm to quantify Glo II activity.
The method was validated for stability, sensitivity, linearity, and interference resistance.

Main Results:

The 2,4-DNPH method demonstrated high stability, sensitivity, and linearity.
It showed strong correlation with the existing UV method for Glo II activity measurement.
The assay proved resistant to interference from various biochemical substances.

Conclusions:

The 2,4-DNPH assay provides a simple, accurate, and cost-effective means to measure Glo II activity.
This novel method is suitable for diverse laboratory settings, including research and clinical diagnostics.
It holds potential for advancing research and applications in diseases linked to Glo II dysregulation.