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Updated: Jun 11, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Diet patterns, gut microbiota and metabolic disorders: Perspectives and challenges
Maria Dalamaga1, Christina Tsigalou2
1Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 75 Mikras Asias, 11527, Athens, Greece.
Diet significantly impacts gut microbiota composition and diversity. Unhealthy diets can lead to reduced microbial diversity, promoting inflammation and metabolic disorders like obesity.
Area of Science:
- Nutrition Science
- Microbiology
- Public Health
Background:
- Obesity and metabolic disorders are global health concerns.
- Gut microbiota alterations are linked to obesity pathogenesis.
- Diet influences gut microbiome composition and host metabolism.
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