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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
Published on: June 13, 2010
Dye self-organization in doped silica nanoparticles increases the electrochemiluminescence emission in magnetic
Yemataw Addis Alemu1, Marinella Difonzo1, Damiano Genovese1
1Dipartimento di Chimica, Giacomo Ciamician - Via F. Selmi 2, 40126 Bologna, Italy. enrico.rampazzo@unibo.it.
Abstract:
We have investigated the effect of dye distribution on the electrochemiluminescence (ECL) intensity measured with a simplified magnetic bead-based immunoassay for two families of silica nanoparticles (NPs) doped with the cationic Ru(bpy)32+ and the zwitterionic Ru(bpy)2bps complexes (bps = bathophenanthroline disulfonate). The NPs doped with the Ru(bpy)2bps complex, which can efficiently self-organize in the NP volume favoring ECL generation, resulted in 150-400% signal enhancement compared to the Ru(bpy)32+-doped ones.

