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Protocol for an open-source system to integrate calcium imaging, pupillometry, and locomotion-estimated tracking in
Maria M Ortiz-Juza1, Jovan Tormes-Vaquerano2, Sophia M Hegel3
1Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
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A wide selection of behavioral assays in systems neuroscience relies on head-fixation protocols to integrate in vivo multi-photon imaging approaches. For this, simultaneous pupillometry and locomotion tracking in head-fixed mice are used to measure behavioral responses and identify neural correlates. Here, we present an open-source protocol for assembling a complete head-fixation system that integrates pupillometry and locomotion-estimated tracking with multi-photon calcium imaging. We include detailed procedures for head-fixation and for data collection.
