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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Jun 7, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Published on: August 9, 2019

RNP particles at splice junction sequences on Drosophila chorion transcripts.

Y N Osheim, O L Miller, A L Beyer

|November 1, 1985

Summary

Specific ribonucleoprotein (RNP) particles bind to nascent RNA transcripts at splice junctions. These RNP particles may play a crucial role in RNA splicing by holding splice sites together during processing.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Heterogeneous nuclear ribonucleoprotein (HnRNP) particles are observed on nascent RNA transcripts.
The functional significance of HnRNP particle binding sites on RNA remains to be fully elucidated.

Purpose of the Study:

To investigate the role of specific sequences bound by HnRNP particles in RNA processing.
To correlate nascent transcript morphology with nucleotide sequences in Drosophila chorion genes.

Main Methods:

Electron microscopic visualization of spread chromatin.
Correlation of nascent transcript morphology with nucleotide sequences of Drosophila chorion s36-1 and s38-1 genes.

Main Results:

HnRNP particles (approx. 25 nm) are localized at the splice junctions of introns in nascent chorion transcripts.

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Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

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Published on: March 28, 2025

A larger particle (approx. 40 nm), possibly formed by particle coalescence, is observed on more mature transcripts near splice junctions.

Conclusions:

The observed RNP structures at splice junctions suggest a role in RNA splicing.
These RNP particles may facilitate the bringing together and maintenance of splice junctions during bipartite splicing.