Related Experiment Video
Updated: Jun 11, 2025
13:25
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
Published on: May 15, 2021
5.6K
CDKN1A promotes Cis-induced AKI by inducing cytoplasmic ROS production and ferroptosis
Qian Gao1, Jun-Ming Chen2, Chen-Sui-Zi Li1
1Department of Pharmacy, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China; School of Medicine, Shanghai Jiao Tong University, Shanghai, 200125, China.
Summary
CDKN1A is identified as a key factor in cisplatin-induced acute kidney injury (AKI). Reducing CDKN1A levels protects kidney cells from damage, suggesting its potential for early AKI diagnosis and treatment.
Area of Science:
- Nephrology
- Molecular Biology
- Bioinformatics
Background:
- Cisplatin chemotherapy can cause acute kidney injury (AKI).
- Early diagnosis and targeted therapies for AKI remain challenging.
- The role of CDKN1A in cisplatin-induced AKI is not well understood.
Purpose of the Study:
- To investigate the role of CDKN1A in cisplatin-induced AKI.
- To evaluate CDKN1A as a potential biomarker for early AKI diagnosis.
- To explore CDKN1A's therapeutic potential in AKI.
Main Methods:
- Bioinformatics analysis of the GSE85957 dataset.
- Machine learning algorithms to identify key genes in cisplatin-induced AKI.
- Experimental validation using human and rat kidney cell lines.
- Quantitative PCR and Western Blotting to assess protein levels.
Main Results:
- CDKN1A was identified as a key gene associated with ferroptosis and the p53 pathway in AKI.
- CDKN1A negatively regulates cell cycle progression (G1/S phase transition).
- Knockdown of CDKN1A reduced cisplatin-induced kidney cell injury, oxidative stress, and ferroptosis.
Conclusions:
- CDKN1A is a promising biomarker for early detection of cisplatin-induced AKI.
- Targeting CDKN1A may offer a novel therapeutic strategy for AKI.
- Integrated bioinformatics and experimental approaches can identify key disease mechanisms and biomarkers.
Related Concept Videos
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
8
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
8
01:22Acute Kidney Injury I: Introduction
2
Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
2
01:46Electron Transport Chain: Complex I and II
12.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.1K
01:25Chronic Kidney Disease I: Introduction
2
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage kidney disease (ESKD). At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate...
2