A plasmonic biosensor pre-diagnostic tool for Familial Mediterranean Fever

Area of Science:

• Biomedical Engineering
• Molecular Diagnostics
• Genetic Disorders

Background:

• Familial Mediterranean Fever (FMF) is an autosomal recessive genetic disorder linked to MEFV gene mutations.
• Current diagnostic methods rely on clinical symptoms, family history, and MEFV gene testing, which can be costly and inconclusive.
• Accurate and early diagnosis of FMF is crucial for timely treatment and improved patient outcomes.

Purpose of the Study:

• To develop and validate a novel biosensor platform for detecting pyrin protein levels in FMF diagnosis.
• To provide a cost-effective and accurate alternative to traditional genetic testing for FMF.
• To enhance early diagnosis and treatment initiation for FMF patients.

Main Methods:

• Utilized gold nanoparticle-based plasmonic chips functionalized with anti-pyrin antibodies.
• Integrated an optofluidic system with visible light spectroscopy for real-time pyrin protein detection.
• Assessed the biosensor's detection limit, specificity, and signal stability.

Main Results:

• The biosensor achieved a low detection limit of 0.24 ng/mL with high specificity for pyrin protein.
• Demonstrated significant differences in pyrin protein levels between healthy individuals and FMF patients.
• The developed system exhibited signal stability for up to six months.

Conclusions:

• The novel biosensor platform offers a promising, cost-effective diagnostic tool for FMF.
• This technology can significantly improve diagnostic accuracy and facilitate earlier treatment for FMF.
• The biosensor represents a valuable advancement in managing Familial Mediterranean Fever.