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Updated: Jun 11, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily
Richard D Schargel1, M Zuhaib Qayyum2, Ajay Singh Tanwar2
1Department of Microbiology, Cornell University, Ithaca, NY, USA. rds278@cornell.edu.
Abstract:
RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.
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