Related Experiment Video
Updated: Jul 26, 2026

Bioassays for Monitoring Insecticide Resistance
Published on: December 30, 2010
Improving Sphenophorus levis Adult Mortality Through Solid Insecticide Applications and Increased Insecticide Dose
Pedro Henrique Urach Ferreira1, Marcelo da Costa Ferreira2, Eliane Vieira3
1Phytosanitary Dept, UNESP, Jaboticabal, SP, Brazil. pedro.esalq@gmail.com.
Abstract:
The sugarcane weevil (Sphenophorus levis Vaurie, 1978) is currently considered the most important sugarcane pest in Brazil, causing significant yield losses. Application methods of insecticides for S. levis control have not been effective, mostly due to the insect's cryptic behavior below the soil surface which suppresses the correct placement of insecticide active ingredients on target. Two experiments were conducted using an innovative bioassay methodology that simulates sugarcane field conditions to effectively evaluate S. levis adult mortality and insecticide residues in the soil under different treatments. The first experiment aimed to assess the efficacy of two liquid- and solid-applied insecticides, while the second aimed to examine the effect of increasing the dose of lambda-cyhalothrin + thiamethoxam on S. levis adult control. The novel bioassays simulated liquid and solid insecticide applications on sugarcane by exposing S. levis adults to chemical residuals on rhizomes and in soil after insecticide application. In the first experiment, low S. levis adult control was detected (< 53% mortality) across all treatments, where both solid and liquid applications of lambda-cyhalothrin + thiamethoxam provided greater efficacy levels than imidacloprid and control treatments, respectively. Solid applications maintained higher insecticide concentrations in the soil for longer periods than liquid insecticide applications, providing maximum insect control levels 7 days after application. The second experiment revealed that solid applications at higher insecticide doses significantly improved control of S. levis adult (76.7% mortality) and resulted in greater insecticide concentrations in the soil compared to the recommended label rate (58.8% mortality).
Related Concept Videos
Pharmaceutical Poisoning: Treatment Strategies
Production of Biopesticides

