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Related Concept Videos

Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

2.0K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
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Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling

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Stable Isotope Tracing Experiments Using LC-MS.

Esther A Zaal1,2, Jeroen W A Jansen3,4, Celia R Berkers3,4

  • 1Division of Cell Biology, Metabolism & Cancer, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. e.a.zaal@uu.nl.

Methods in Molecular Biology (Clifton, N.J.)
|October 1, 2024
PubMed
Summary
This summary is machine-generated.

Metabolomics research uses isotope tracing to dynamically map cellular metabolism, crucial for understanding diseases like cancer and developing new therapies. This method reveals metabolic rewiring for improved diagnostics and drug responses.

Keywords:
FluxomicsHigh-performance liquid chromatographyIsotope tracingMass spectrometryMetabolomics

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Area of Science:

  • Biochemistry and Molecular Biology
  • Systems Biology
  • Metabolomics

Background:

  • Metabolomics is crucial for understanding cellular functions and disease mechanisms.
  • Metabolic alterations are key drivers in diseases such as cancer, influencing progression and therapeutic outcomes.
  • Identifying metabolic rewiring is vital for developing novel diagnostic and therapeutic strategies.

Purpose of the Study:

  • To outline a robust isotope tracing protocol for analyzing cellular metabolism.
  • To provide a valuable resource for researchers using isotopic tracing in cell culture models.
  • To detail the application of high-resolution mass spectrometry coupled with liquid chromatography for metabolic studies.

Main Methods:

  • Utilizing isotope tracing to dynamically track labeled substrates through cellular biochemical reactions.
  • Employing high-resolution mass spectrometry coupled to liquid chromatography for sensitive detection.
  • Applying the protocol in cell culture-based models for comprehensive analysis.

Main Results:

  • The study provides a detailed protocol for isotope tracing experiments.
  • It covers essential aspects of experimental design and data analysis.
  • The described methodology enables a dynamic understanding of cellular metabolic pathways.

Conclusions:

  • Isotope tracing is a powerful technique for elucidating cellular metabolic wiring.
  • The outlined protocol facilitates the investigation of metabolic rewiring in disease contexts.
  • This approach supports the discovery of new diagnostic and therapeutic strategies in metabolomics.