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Related Experiment Video

Updated: Jul 12, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Differential Mobility Spectrometry-Based Cardiolipin Analysis.

Fabien Riols¹, Michael Witting^1,2, Mark Haid³

¹Metabolomics and Proteomics Core, Helmholtz Zentrum München, Neuherberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2024

Summary

This study introduces a new method for quantifying cardiolipins (CL), essential mitochondrial lipids. This technique aids in understanding mitochondrial function and related diseases.

Keywords:

Cardiolipins Differential mobility spectrometry Flow injection analysis Mass spectrometry Mitochondria

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Last Updated: Jul 12, 2026

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Published on: April 17, 2017

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

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Shotgun Lipidomics of Rodent Tissues

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Published on: November 18, 2022

Area of Science:

Biochemistry
Cell Biology
Analytical Chemistry

Background:

Cardiolipins (CL) are unique lipids with four fatty acids, primarily found in mitochondria.
They are crucial for mitochondrial integrity, shape, and metabolic pathways.
Mitochondrial dysfunction and disease are linked to altered CL composition.

Purpose of the Study:

To develop and validate an analytical method for quantifying cardiolipins.
To enable detailed study of the mitochondrial lipidome, specifically CL.
To support research into CL-related diseases.

Main Methods:

Utilizing flow injection analysis coupled to differential mobility spectrometry (FIA-DMS).
Applying DMS-based shotgun lipidomics for lipid quantification.
Analyzing CL at the sum composition level in isolated mitochondria and liver tissue.

Main Results:

Successful quantification of cardiolipins in biological samples.
Demonstrated the utility of FIA-DMS for CL analysis.
Provided a method for studying mitochondrial CL composition.

Conclusions:

The developed FIA-DMS method is effective for quantifying cardiolipins.
This technique advances the study of mitochondrial lipidomics.
It offers a valuable tool for investigating CL in health and disease.