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Related Experiment Videos

Glucocorticoid hormone binding to human adipose tissue.

M Rebuffé-Scrive, K Lundholm, P Björntorp

    European Journal of Clinical Investigation
    |October 1, 1985
    PubMed
    Summary

    Human adipose tissue exhibits specific binding for triamcinolone, a glucocorticoid. Binding site characteristics and affinity are similar to other tissues, with notable individual variations and regional differences in fat tissue.

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    Area of Science:

    • Endocrinology
    • Molecular Pharmacology
    • Adipose Tissue Biology

    Background:

    • Glucocorticoid receptors are crucial in regulating various physiological processes.
    • Understanding triamcinolone binding in human adipose tissue is important for metabolic research.
    • Adipose tissue plays a significant role in systemic glucocorticoid metabolism and action.

    Purpose of the Study:

    • To characterize the specific binding of triamcinolone in human adipose tissue cytosol.
    • To compare binding parameters with those reported in other tissues and species.
    • To investigate potential differences in binding between omental and subcutaneous adipose depots.

    Main Methods:

    • Cytosolic preparations from human adipose tissue obtained during surgery were used.
    • Radioligand binding assays were performed to assess triamcinolone binding.
    • Competitive inhibition studies were conducted using various unlabeled steroids.

    Main Results:

    • Specific, saturable binding of triamcinolone was identified in human adipose tissue cytosol.
    • Binding affinity and site concentration were comparable to glucocorticoid binding in other tissues.
    • Triamcinolone binding was significantly higher in omental compared to subcutaneous adipose tissue per unit protein and cell surface area.

    Conclusions:

    • Human adipose tissue possesses specific binding sites for triamcinolone, consistent with glucocorticoid receptor activity.
    • Significant inter-individual variability in binding characteristics was observed.
    • Regional differences in triamcinolone binding exist between omental and subcutaneous adipose depots.

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