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Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos.

Laura Bel Borja¹, Samuel J P Taylor¹, Flavie Soubigou¹

¹Molecular, Cellular and Developmental Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.

Journal of Cell Science

|October 2, 2024

Summary

This summary is machine-generated.

Inner kinetochore Polo-like kinase 1 (PLK-1), targeted by CENP-C, plays a distinct role in chromosome congression during mitosis. This interaction is crucial for proper kinetochore function and cell division.

Keywords:

CCAN CENP-C Kinetochore MIS12 Mitosis PLK

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Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Polo-like kinase 1 (PLK-1) is vital for cell division, influencing spindle assembly, chromosome segregation, and cytokinesis.
PLK-1 localizes to centrosomes, nuclear envelope, and kinetochores, with distinct roles at outer and inner kinetochore regions.

Purpose of the Study:

To investigate the specific, non-redundant function of inner kinetochore-targeted PLK-1.
To elucidate the role of the CENP-C/PLK-1 interaction in the Caenorhabditis elegans embryo.

Main Methods:

Utilized the Caenorhabditis elegans embryo model system.
Studied the interaction between CENP-C and PLK-1 during prometaphase and metaphase.
Assessed the impact of disrupting the CENP-C-PLK-1 interaction on kinetochore components and chromosome congression.

Main Results:

CENP-C targets PLK-1 to the inner kinetochore during prometaphase and metaphase.
Disruption of the CENP-C-PLK-1 interaction caused kinetochore component imbalance and chromosome congression defects.
CDC-20 recruitment remained unaffected upon disruption of the CENP-C-PLK-1 interaction.

Conclusions:

Inner kinetochore PLK-1 recruitment by CENP-C has functions distinct from outer kinetochore PLK-1.
The CENP-C-PLK-1 interaction is essential for proper chromosome congression, independent of CDC-20.
Findings provide insights into the multifaceted roles of PLK-1 in mitotic processes.