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CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos.
Laura Bel Borja1, Samuel J P Taylor1, Flavie Soubigou1
1Molecular, Cellular and Developmental Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
Journal of Cell Science
|October 2, 2024
Summary
Inner kinetochore Polo-like kinase 1 (PLK-1), targeted by CENP-C, plays a distinct role in chromosome congression during mitosis. This interaction is crucial for proper kinetochore function and cell division.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Polo-like kinase 1 (PLK-1) is vital for cell division, influencing spindle assembly, chromosome segregation, and cytokinesis.
- PLK-1 localizes to centrosomes, nuclear envelope, and kinetochores, with distinct roles at outer and inner kinetochore regions.
Purpose of the Study:
- To investigate the specific, non-redundant function of inner kinetochore-targeted PLK-1.
- To elucidate the role of the CENP-C/PLK-1 interaction in the Caenorhabditis elegans embryo.
Main Methods:
- Utilized the Caenorhabditis elegans embryo model system.
- Studied the interaction between CENP-C and PLK-1 during prometaphase and metaphase.
- Assessed the impact of disrupting the CENP-C-PLK-1 interaction on kinetochore components and chromosome congression.
Main Results:
- CENP-C targets PLK-1 to the inner kinetochore during prometaphase and metaphase.
- Disruption of the CENP-C-PLK-1 interaction caused kinetochore component imbalance and chromosome congression defects.
- CDC-20 recruitment remained unaffected upon disruption of the CENP-C-PLK-1 interaction.
Conclusions:
- Inner kinetochore PLK-1 recruitment by CENP-C has functions distinct from outer kinetochore PLK-1.
- The CENP-C-PLK-1 interaction is essential for proper chromosome congression, independent of CDC-20.
- Findings provide insights into the multifaceted roles of PLK-1 in mitotic processes.
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