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Updated: Jun 11, 2025
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Coalescence of liquid or gel-like DNA-encapsulating microdroplets
Takashi Nishio1, Helmut Schiessel1,2
1Cluster of Excellence Physics of Life, TUD Dresden University of Technology, 01307 Dresden, Germany.
Abstract:
Liquid-liquid phase separation plays a prominent role in the physics of life, providing the cells with various membrane-less compartments. These structures exhibit a range of material properties that, in many cases, change over time. Inspired by this, we investigate here an aqueous two-phase system formed by mixing polyethylene glycol with dextran. We modulate the material properties of the resulting dextran droplets by adding DNA that readily enters the droplets. We find a non-monotonic dependence of the physical properties of the droplets under the imposed ionic conditions.
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