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Serotypes of bovine astrovirus
Journal of Clinical Microbiology
|October 1, 1985
Summary
Bovine astrovirus isolates show distinct serotypes with shared antigens. One isolate exhibited similar cell tropism and pathology, indicating potential for further classification of bovine astrovirus serotypes.
Area of Science:
- Veterinary Virology
- Immunology
- Gastroenterology
Background:
- Bovine astrovirus (BAV) is an enteric pathogen affecting cattle.
- Understanding BAV diversity is crucial for disease control and vaccine development.
- Previous studies have identified limited BAV serotypes and their associated pathology.
Purpose of the Study:
- To characterize antigenicity and serotypic diversity among BAV isolates.
- To investigate the cell tropism and cytopathology of different BAV serotypes.
- To assess the prevalence of distinct BAV serotypes in field isolates.
Main Methods:
- Immunofluorescence assays to detect common BAV antigens.
- Neutralization assays for strain-specific antigen identification and serotyping.
- Cell culture studies to observe cell tropism and cytopathology in M cells of Peyer's patches.
Main Results:
- Three BAV isolates (UK, US1, US2) demonstrated common and strain-specific antigens.
- At least two, possibly three, distinct BAV serotypes were identified among the tested isolates.
- Isolate US2 shared restrictive cell tropism and cytopathology with isolate US1, affecting M cells of the Peyer's patches.
- Serotyping of 16 field isolates revealed the existence of additional, undefined serotypes.
Conclusions:
- Bovine astrovirus exhibits significant serotypic diversity.
- Shared cell tropism and cytopathology can occur across different BAV serotypes.
- Further research is needed to fully characterize the spectrum of BAV serotypes and their epidemiological impact.
