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Area of Science:

  • Agricultural science
  • Plant biochemistry
  • Spectroscopy

Background:

  • Traditional antioxidant analysis of holy basil is time-consuming.
  • Hyperspectral imaging offers a rapid, non-destructive method for plant property assessment.
  • Phytochemical quantification, including phenolic content, is crucial for understanding plant properties.

Purpose of the Study:

  • To develop a rapid, non-destructive method for determining total phenolic content in Thai holy basil using hyperspectral imaging and machine learning.
  • To classify phenolic content levels into 'low' and 'normal-to-high' categories.
  • To evaluate the performance of a neural network model against other machine learning techniques.

Main Methods:

  • Acquired hyperspectral data from 26 holy basil cultivars across different growth stages.
  • Extracted 22 statistical features in time and frequency domains from spectral data.
  • Developed and validated a neural network model for phenolic content classification.

Main Results:

  • The neural network model achieved an area under the receiver operating characteristic curve of 0.8113.
  • The model demonstrated superior performance compared to other machine learning techniques.
  • The model showed increased confidence in predicting phenolic content for older holy basil samples.

Conclusions:

  • Hyperspectral imaging integrated with feature extraction and machine learning provides an effective tool for rapid, non-destructive phenolic content assessment in holy basil.
  • This approach has the potential to streamline the screening of antioxidant properties in plants.
  • The study facilitates efficient decision-making for researchers through comprehensive spectral data analysis.