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A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
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Comprehensive analysis of the expression and prognosis for cyclin-dependent protein kinase family in osteosarcoma

Jianshui Mao1, Hui-Min Li1, Zhidan Huang1

  • 1Department of Orthopedics, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang, P R China.

Nucleosides, Nucleotides & Nucleic Acids
|October 2, 2024
PubMed
Abstract

Insights

Cyclin-dependent protein kinases (CDKs) show varied expression in osteosarcoma, with specific CDKs (3, 6, 9, 18) identified as potential therapeutic targets. These CDKs may influence immune checkpoints and m6A modifications in cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cyclin-dependent protein kinases (CDKs) are crucial for cancer cell survival and growth, making them promising therapeutic targets.
  • Limited research exists on CDK expression profiles and prognostic significance in osteosarcoma.

Purpose of the Study:

  • To investigate differential CDK expression in osteosarcoma.
  • To identify prognostic biomarkers among CDKs for osteosarcoma patients.
  • To explore the relationship between CDKs, microRNAs (miRNAs), immune checkpoints, and m6A-related genes in osteosarcoma.

Main Methods:

  • Gene and microRNA expression data for osteosarcoma were analyzed using datasets from the Gene Expression Omnibus (GEO) and TARGET databases.
  • A network of miRNAs and CDKs was constructed using Cytoscape.
  • Functional and pathway enrichment analyses were performed.
  • Interactions between CDKs, immune checkpoint genes, and m6A-related genes were examined.

Main Results:

  • CDK1-5, 16-18, and 17 showed significantly higher expression in osteosarcoma compared to normal samples.
  • CDK7-9, 11B, 16, and 20 exhibited significantly lower expression in osteosarcoma.
  • Low expression of CDK3 and 18, or high expression of CDK6 and 9, correlated with a favorable prognosis and longer survival.
  • A significant association was observed between CDK expression, immune checkpoint genes, and m6A-related genes.

Conclusions:

  • CDK3, 6, 9, and 18 are identified as potential therapeutic targets for osteosarcoma.
  • CDKs play a role in regulating m6A modifications and immune checkpoints in osteosarcoma cells.
Keywords:
Osteosarcomacyclin-dependent protein kinase familyimmune checkpointm6AmiRNAsprognosis

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