Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

506
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
506
Qualitative Analysis01:10

Qualitative Analysis

230
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
230
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

733
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
733
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.0K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.0K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

499
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
499
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

2.5K
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectroscopic and molecular modeling methods to investigate the interaction between psycho-stimulant modafinil and calf thymus DNA using ethidium bromide as a fluorescence probe.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2022
Same author

Exploring Allosteric Signaling in the Exit Tunnel of the Bacterial Ribosome by Molecular Dynamics Simulations and Residue Network Model.

Frontiers in molecular biosciences·2020
Same author

Multispectroscopic and Computational Investigation of ct-DNA Binding Properties with Hydroxybenzylidene Containing Tetrahydrocarbazole Derivative.

Journal of fluorescence·2018
Same author

Identification of potential allosteric communication pathways between functional sites of the bacterial ribosome by graph and elastic network models.

Biochimica et biophysica acta. General subjects·2017
Same author

Investigation of binding properties of two ethidium derivatives with serum albumins: spectral and computational approach.

Journal of biomolecular structure & dynamics·2017
Same author

Distance-dependent intrinsic fluorescence of proteins on aluminum nanostructures.

Proceedings of SPIE--the International Society for Optical Engineering·2013

Related Experiment Video

Updated: Jun 11, 2025

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products
08:07

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Published on: May 22, 2019

11.0K

Spectroscopic techniques and chemometrics for ink analysis: Document analysis application.

Ummugulsum Mirzanli1, Pelin Guzel2, Nuriye Akbay3

  • 1Division of Forensic Sciences, Institute of Postgraduate Education, Istanbul Medeniyet University, 34700 Istanbul, Turkey.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 2, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a combined spectroscopic and chemometric method to detect falsified documents using ballpoint pen inks. The methodology, utilizing UV-Vis and FTIR-ATR spectroscopy, effectively identifies ink brands and detects forgeries even with limited sample sizes.

Keywords:
ChemometricsForensic sciencesHierarchical cluster analysisInks analysisPrincipal component analysis

More Related Videos

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks
10:31

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Published on: May 8, 2015

13.7K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

4.9K

Related Experiment Videos

Last Updated: Jun 11, 2025

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products
08:07

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Published on: May 22, 2019

11.0K
Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks
10:31

Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

Published on: May 8, 2015

13.7K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

4.9K

Area of Science:

  • Forensic Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Document forgery is a significant challenge in forensic investigations.
  • Reliable methods are needed to authenticate documents and identify counterfeit items.
  • Spectroscopic techniques offer non-destructive analysis for material characterization.

Purpose of the Study:

  • To develop and validate a combined spectroscopic and chemometric methodology for detecting falsified documents.
  • To assess the discriminatory power of UV-Vis and FTIR-ATR spectroscopy for ballpoint pen ink analysis.
  • To demonstrate the application of the methodology in real-world forensic scenarios, including limited sample analysis.

Main Methods:

  • Application of UV-Vis and FTIR-ATR spectroscopy combined with Principal Component Analysis (PCA).
  • Analysis of eleven ballpoint pen ink samples (black, blue, red) from three brands in Türkiye.
  • Development of a dried sample preparation technique to enhance FTIR-ATR sensitivity for small samples.

Main Results:

  • FTIR-ATR spectroscopy exhibited higher discriminative power than UV-Vis for identifying ink brands.
  • UV-Vis spectroscopy effectively distinguished inks based on color.
  • The methodology successfully classified ballpoint inks and identified a falsified old-dated check sample.
  • FTIR-ATR analysis confirmed forgery detection from minimal sample amounts.

Conclusions:

  • The proposed methodology, integrating UV-Vis and FTIR-ATR spectroscopy with chemometrics, is effective for classifying and identifying ballpoint inks.
  • The technique is valuable for forensic applications, particularly in detecting document forgery from limited sample quantities.
  • This comprehensive approach is applicable even in resource-limited laboratories with basic spectroscopic capabilities.