Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The relationship between HLA antigens and Bermuda grass hayfever.

Immunogenetics·2011
Same author

Attributes of gammadelta intraepithelial lymphocytes as suggested by their transcriptional profile.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Characterizations of highly expressed genes of four fast-growing bacteria.

Journal of bacteriology·2001
Same author

Detecting anomalous gene clusters and pathogenicity islands in diverse bacterial genomes.

Trends in microbiology·2001
Same author

Type A behavior and coronary atherosclerosis.

Atherosclerosis·2001
Same author

Genomics. Annotation of the Drosophila genome.

Nature·2001

Related Experiment Video

Updated: Jul 23, 2026

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
07:58

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Published on: March 6, 2019

DNA sequence patterns in human, mouse, and rabbit immunoglobulin kappa-genes.

S Karlin, G Ghandour

    Journal of Molecular Evolution
    |January 1, 1985
    PubMed
    Summary

    Comparing DNA patterns in human, mouse, and rabbit immunoglobulin kappa-gene J-C regions reveals species-specific sequence features. These findings highlight variations in gene regulation and duplication, crucial for understanding immune system evolution.

    More Related Videos

    Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
    08:51

    Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

    Published on: March 15, 2019

    In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
    06:21

    In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

    Published on: April 25, 2019

    Related Experiment Videos

    Last Updated: Jul 23, 2026

    qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
    07:58

    qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

    Published on: March 6, 2019

    Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
    08:51

    Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

    Published on: March 15, 2019

    In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
    06:21

    In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

    Published on: April 25, 2019

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Comparative Genomics

    Background:

    • Immunoglobulin kappa-gene (J-C regions) play a critical role in antibody diversity.
    • Understanding DNA sequence patterns is key to deciphering gene regulation and function.
    • Comparative analysis across species reveals evolutionary conserved and divergent genetic elements.

    Purpose of the Study:

    • To compare DNA sequence patterns within the J-C regions of human, mouse, and rabbit immunoglobulin kappa-genes.
    • To identify and analyze specific DNA motifs such as dyad symmetry, nucleotide runs, and repeat clusters.
    • To investigate the potential functional implications of these patterns on gene regulation and V-J segment interactions.

    Main Methods:

    • Comparative sequence analysis of immunoglobulin kappa-gene J-C regions from human, mouse, and rabbit.
    • Identification and characterization of DNA patterns including dyad symmetry, nucleotide runs, repeat clusters, and high-frequency oligonucleotides.
    • Bioinformatic analysis to assess the significance and distribution of identified DNA patterns.

    Main Results:

    • Significant dyad symmetry pairings were observed within the J5-C intron of all species, emphasizing control-enhancer elements.
    • Dyad symmetry between J-C and V-gene domains suggests varying affinities between V and J segments, with the consensus heptamer showing longer combinations.
    • Rabbit sequences exhibit extensive runs and repeat clusters indicating high duplication, absent in human and mouse sequences.
    • High-frequency oligonucleotides, particularly the consensus nonamer, were found 5' to J segments, notably in mouse sequences.

    Conclusions:

    • Species-specific DNA patterns exist within immunoglobulin kappa-gene J-C regions, influencing gene regulation.
    • Dyad symmetry and repeat structures play distinct roles in gene organization and evolutionary divergence across mammals.
    • The findings provide insights into the mechanisms of V-J recombination and immune gene evolution.