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Related Experiment Videos

Cytoplasmic IgM in leukemic B cells by flow cytometry.

K C Loftin, J M Reuben, E M Hersh

    Leukemia Research
    |January 1, 1985
    PubMed
    Summary

    This study developed a flow cytometry method to detect intracellular cytoplasmic immunoglobulin M (IgM) in leukemia cells. The method successfully identified cytoplasmic IgM in specific leukemia types, aiding in their characterization.

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    Area of Science:

    • Hematology
    • Immunology
    • Cell Biology

    Background:

    • Intracellular cytoplasmic immunoglobulin M (IgM) presence in leukemia requires accurate detection methods.
    • Flow cytometry offers a potential avenue for reproducible assessment of cytoplasmic IgM.

    Purpose of the Study:

    • To establish a reliable flow cytometry technique for detecting intracellular cytoplasmic IgM in leukemic cells.
    • To investigate the expression of cytoplasmic IgM in various leukemia subtypes and cell lines.

    Main Methods:

    • Utilized flow cytometry to analyze cytoplasmic IgM expression.
    • Employed a method involving blocking surface membrane IgM, followed by fixation and staining for intracellular IgM.
    • Used Daudi (Burkitt's lymphoma) and Nalm-6 (pre-B ALL) cell lines as prototypes, with normal B cells and CML cells as controls.

    Main Results:

    • The developed flow cytometry method reliably detected cytoplasmic IgM in Nalm-6 cells (77.3% +/- 7.5%).
    • Normal B cells and CALLA+ ALL/CML cells showed no detectable cytoplasmic IgM after surface blocking.
    • Significant cytoplasmic IgM expression was observed in chronic lymphocytic leukemia (CLL) patients (7.8-76.7%) and Daudi cells (45.7-89.3%).

    Conclusions:

    • Flow cytometry provides an efficient and rapid method for demonstrating cytoplasmic IgM in leukemic cells.
    • This technique is valuable for characterizing cell lines like Nalm-6 and Daudi, and for diagnosing CLL.

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