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Area of Science:

  • Neuroscience
  • Biophysics
  • Chemical Biology

Background:

  • Voltage imaging complements traditional electrophysiology for cellular physiology.
  • Silicon rhodamine dyes (BeRST) are explored for voltage imaging.
  • Existing methods like Ca2+ imaging do not directly visualize bioelectricity.

Purpose of the Study:

  • To investigate the impact of structural modification on BeRST dye properties.
  • To synthesize and characterize a new BeRST derivative, diEt BeRST.
  • To evaluate diEt BeRST's performance in neuronal voltage imaging.

Main Methods:

  • Chemical modification of BeRST to diEt BeRST by altering aniline groups.
  • Quantification of voltage sensitivity (% ΔF/F per 100 mV).
  • Assessment of cellular brightness and solubility.
  • Recording of spontaneous and evoked action potentials in neurons.
  • Simultaneous voltage and Ca2+ imaging in hippocampal neurons.

Main Results:

  • diEt BeRST exhibits a voltage sensitivity of 40% ΔF/F per 100 mV, a 33% increase over BeRST.
  • Cellular brightness of diEt BeRST is 20% of BeRST due to lower solubility.
  • diEt BeRST successfully records action potentials from multiple neurons in single trials.
  • Simultaneous imaging reveals neuronal activity patterns not resolvable by Ca2+ imaging alone.

Conclusions:

  • Structural modification of BeRST dyes can enhance voltage sensitivity.
  • diEt BeRST is a promising tool for advanced neuronal voltage imaging.
  • This work provides insights into structure-property relationships for silicon rhodamine voltage indicators.