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Mesenchymal stem cells suppress inflammation by downregulating interleukin-6 expression in intestinal perforation
Eko Setiawan1, Agung Putra2,3,4, Dimas Irfan Nabih5
1Department of Surgery, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang.
Introduction:
Intestinal perforation has significant fatality due to sepsis contamination and prolonged inflammation. Studies showed that mesenchymal stem cells (MSCs) secreted cytokines and growth factors to reduce inflammation. This study aims to reveal the role of MSCs in controlling inflammation in intestinal perforation wound healing by measuring interleukin-6 (IL-6) and leukocytes in injured tissue.
Materials And Methods:
A total of 48 rat models with a 10-mm longitudinal incision at the small intestine were divided into four groups: sham, control, Treatment group 1 (T1) injected with MSC doses of 1.5×106 cells and Treatment group 2 (T2) with 3×106 cells. IL-6 expressions were determined using western blot analysis, whereas the leukocyte infiltrations were assessed using the histopathological examination. All variables were evaluated on day 3 and 7.
Results:
Leukocyte infiltration is significantly lower in T1 and T2 compared to control group in day 3 and 7 (P<0.05), while there were no differences between the two treatment groups. The expression of IL-6 was found to be significantly lower in the T1 and T2 groups compared to the control group on days 3 and 7 (P<0.05), with no significant differences observed between the two treatment groups.
Conclusion:
MSCs administration in rats with intestinal perforation reduced inflammation by controlling leukocyte infiltration and IL-6 expression.
Insights
Mesenchymal stem cells (MSCs) reduce inflammation in intestinal perforation by decreasing interleukin-6 (IL-6) and leukocyte infiltration. This study demonstrates MSCs
Area of Science:
- Regenerative Medicine
- Immunology
- Gastroenterology
Background:
- Intestinal perforation leads to high mortality due to sepsis and inflammation.
- Mesenchymal stem cells (MSCs) secrete factors that can modulate inflammation.
- Understanding MSCs' role in intestinal injury is crucial for developing new treatments.
Purpose of the Study:
- To investigate the efficacy of MSCs in controlling inflammation during intestinal perforation wound healing.
- To quantify the impact of MSCs on interleukin-6 (IL-6) and leukocyte levels in injured intestinal tissue.
Main Methods:
- Establishment of a rat model for intestinal perforation.
- Administration of two different doses of MSCs (1.5×10^6 and 3×10^6 cells) to treatment groups.
- Measurement of IL-6 expression via Western blot and leukocyte infiltration through histopathological examination on days 3 and 7 post-injury.
Main Results:
- Both MSC treatment groups showed significantly reduced leukocyte infiltration compared to the control group on days 3 and 7.
- Interleukin-6 (IL-6) expression was significantly lower in MSC-treated groups versus the control group on days 3 and 7.
- No significant differences in IL-6 expression or leukocyte infiltration were observed between the two MSC treatment groups.
Conclusions:
- MSC administration effectively reduces inflammation in a rat model of intestinal perforation.
- MSCs control inflammation by suppressing leukocyte infiltration and IL-6 expression.
- These findings highlight the therapeutic potential of MSCs for managing intestinal perforation complications.
