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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
Published on: August 11, 2008
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Multidrug-resistant tuberculosis clusters and transmission in Taiwan: a population-based cohort study
Kuang-Hung Liu1,2, Yu-Xin Xiao1,2, Ruwen Jou1,2
1Tuberculosis Research Center, Centers for Disease Control, Ministry of Health and Welfare, Taipei, Taiwan.
Frontiers in Microbiology
|October 3, 2024
Summary
Multidrug-resistant tuberculosis (MDR-TB) genotyping using whole genome sequencing identified transmission clusters and outbreaks in Taiwan. Compensatory mutations in MDR-TB strains can serve as epidemiological markers to interrupt transmission.
Area of Science:
- Genomics
- Epidemiology
- Microbiology
Background:
- Multidrug-resistant tuberculosis (MDR-TB) poses a significant challenge to Taiwan's TB control program.
- A substantial proportion of MDR-TB cases are newly diagnosed, highlighting the need for effective contact tracing and outbreak detection.
Purpose of the Study:
- To investigate the genetic diversity and transmission patterns of MDR-TB in Taiwan using whole genome sequencing (WGS).
- To identify transmission clusters and outbreaks of MDR-TB and explore potential epidemiological markers for transmission interruption.
Main Methods:
- A population-based retrospective cohort study analyzed MDR-TB cases from 2019 to 2022.
- Whole genome sequencing (WGS) was performed on isolates, and data were analyzed using TB Profiler with phylogenetic methods to identify transmission clusters based on a single nucleotide polymorphism (SNP) threshold.
- Outbreaks were confirmed using genomic data combined with epidemiological links.
Main Results:
- The predominant MDR-TB strain was sublineage 2.2 modern Beijing (48.8%).
- Phylogenetic analysis revealed 20 clusters (25.3% of isolates), with two confirmed outbreaks (one household, one community).
- Males and infections with sublineage 2.1-proto-Beijing genotype showed higher transmission risk. Compensatory mutations, particularly in rpoB and rpoC genes, were associated with clustering, especially in Beijing genotype strains.
Conclusions:
- Routine WGS-based surveillance is recommended for early detection and delineation of MDR-TB transmission clusters.
- Compensatory mutations in Mycobacterium tuberculosis can be utilized as epidemiological markers to aid in interrupting MDR-TB transmission.
Keywords:
Mycobacterium tuberculosismultidrug-resistancemutationtransmissiontuberculosiswhole genome sequencingMore Related Videos
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