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Related Experiment Video

Updated: Jun 14, 2026

Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
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Membrane Tension Regulation is Required for Wound Repair.

Nikita Raj1, Martin S Weiß2, Bart E Vos3

  • 1Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation (ZMBE), Multiscale Imaging Centre, Cells in Motion Interfaculty Center, University of Münster, 48149, Münster, Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|October 3, 2024
PubMed
Summary
This summary is machine-generated.

Cell membrane repair involves coordinated exocytosis and clathrin-mediated endocytosis. Membrane tension acts as a universal trigger, regulating these processes for efficient wound healing and restoring cell function.

Keywords:
Ca2+cellular tensionclathrin‐mediated endocytosisplasma membrane resealing

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Area of Science:

  • Cell Biology
  • Membrane Biology

Background:

  • Eukaryotic plasma membrane disruptions require repair for cell survival.
  • Mechanisms of membrane wound resealing and homeostasis restoration are complex.

Purpose of the Study:

  • To investigate the role of clathrin-mediated endocytosis in plasma membrane wound repair.
  • To elucidate the spatio-temporal coordination of exocytosis and endocytosis during repair.
  • To identify key regulators of membrane wound repair processes.

Main Methods:

  • Induction of plasma membrane wounds.
  • Observation of clathrin-mediated endocytosis at later repair stages.
  • Analysis of early endosome exocytosis at wound sites.
  • Modulation of cytoskeletal elements, membrane tension, and membrane area.

Main Results:

  • Clathrin-mediated endocytosis occurs after initial wound resealing.
  • Compensatory endocytosis is linked to prior early endosome exocytosis sites.
  • Membrane tension is a critical regulator of wounding-associated exo- and endocytic events.
  • Exocytosis and endocytosis are spatio-temporally coordinated during repair.

Conclusions:

  • Membrane tension changes universally trigger plasma membrane wound repair.
  • Early endosome exocytosis is crucial for initial resealing.
  • Clathrin-mediated endocytosis restores homeostasis post-resealing.
  • Coordinated exo- and endocytosis ensures efficient membrane repair.