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Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: Jul 5, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Publisher's Note: "Low-cost device for ratiometric fluorescence measurements" [Rev. Sci. Instrum. 70, 4466-4470

Yordan Kostov¹, Govind Rao^1,2

¹Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA.

The Review of Scientific Instruments

|October 3, 2024

Summary

No abstract available in PubMed .

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