Electrochemical sensor for glutathione reductase based on screen-printed electrodes coated with 3,5-dinitrobenzoic
Anabel Villalonga1, David López-López1, Esther García-Díez1
1Nanosensors and Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
Abstract:
The preparation of a hybrid nanomaterial is reported by covalently attaching 3,5-dinitrobenzoic acid groups to the surface of oxidized multi-walled carbon nanotubes using 1,6-diaminohexane as cross-linking agent. This nanomaterial, modified with the redox mediator, was used as transduction element to construct an amperometric sensor for the efficient indirect determination of glutathione reductase at a low working potential of - 0.05 V, through the oxidation of unconsumed nicotinamide adenine dinucleotide phosphate (NADPH) in the enzymatic reaction. The sensor exhibited an excellent linear response in the range 1.6 to 174 µU/µL, with high reproducibility and selectivity. The developed device was successfully validated in real samples, accurately determining the active enzyme in diluted human serum, making it a promising alternative for the determination of glutathione reductase and other related NADPH-dependent enzymes with relevance in clinical analysis.
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